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The most widely used diagnostic approach is direct detection of Fasciola eggs, by light microscopic examination of stool or of duodenal or biliary aspirates. However, egg production typically does not start until 3 to 4 months after the exposure, whereas antibodies to the parasite may become detectable 2 to 4 weeks postexposure. (See below.) Even during the chronic phase of infection, more than one stool specimen may need to be examined to find the parasite, especially in people with light infections..

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