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Jersey cows were in their 3rd and 4th lactation around 100 days in milk whereas Normande cow was in its 2nd lactation around 180 days in milk. Mammary tissue biopsies were performed using a 9 gauge, 12 cm probe with a 12 mm aperture as described by VanKlompenberg et al.23 in Jersey cows and 70 4mm rotating stainless steel cannula in Normande cow24. A 5 mm incision was made through the skin and connective tissue approximately half way up the rear udder quarter using a scalpel blade before the parenchyma was penetrated with a Hologic ATEC Biopsy Console Handpiece for excision of a tissue core (Hologic Inc., Bedford, MA) and Farr's Instrument.
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